Detection limit was further assessed for one of these highly specific assays.ī. All three assays target chromosomal markers located within the lambdaBa03 prophage region (P元, BA5345, and BA5357). Three assays performed adequately, yielding no false positive or negative results. An interlaboratory ring trial among five European laboratories was then performed to evaluate six assays, including the WHO recommended procedures, using a collection of 90 Bacillus strains. Of the 35 investigated PCR assays, only 4 were 100% specific for the B. thuringiensis strains were often observed. anthracis, cross-reactions with closely related B. Although many of the chromosomal targets evaluated are claimed to be specific to B. Published primer and probe sequences were compared for specificity against 134 available Bacillus spp.
Following a review of the literature, an in silico analysis of all signature sequences reported for identification of B. For the detection of chromosomal DNA, different genes have been used, such as BA813, rpoB, gyrA, plcR, S-layer, and prophage-lambda. While most of the developed assays rely on unique markers present on virulence plasmids pXO1 and pXO2, relatively few assays incorporate chromosomal DNA markers due to the close relatedness of B. There are many PCR-based assays in use for the detection of B. Bacillus anthracis, the causative agent of anthrax, is a zoonotic pathogen that is relatively common throughout the world and may cause life threatening diseases in animals and humans.
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